Serological Technique: Immunotherapy (Contd.). Immunization against Rabies

The vaccine.??The Semplc vaccine is used in India and in most other countries. In India, it is a 5 per cent suspension of the brain of a sheep, paralysed after infection with fixed virus of rabies through cisterna puncture. The brain is removed with sterile precautions, ground into 8 per cent suspension with normal saline and phenolized to obtain a concentration of 1 per cent of the antiseptic. The phenolized suspension is left at 37?C. for 24 hours. Next day enough normal saline is added to reduce the brain substance to .5 per cent (and the phenol to about 0.6 per cent). The suspension is then tested for the presence of phenol and for bacterial sterility. The phenol should produce colour with tinct. ferri perchloride (B.P.) and bacteria should be absent in an aerobic and